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NMI is a distributed infrastructure for microscopy in life sciences with nodes at KTH, and Stockholm, Gothenburg, and Umeå Universities. NMI provides open access to state-of-the-art equipment and expertise in microscopy, and coordinates the participation in international microscopy infrastructures (EuroBioImaging).Technologies available at NMI include: correlative and multimodal light and electron microscopy, intravital microscopy, super-resolution microscopy and spatial proteomics. The techniques are used in fundamental and translational research for studies down to the molecular level in both living and fixed cells and whole organisms. The advanced imaging techniques not only require significant investments in equipment from the host universities but also skilled scientific staff with expertise to operate the advanced microscopes and optimize the analysis.A project portal support execution of projects (www.nmisweden.se). Applications for projects are done via the portal where support also is given to select the most appropriate technique. Project prioritization is primarily based on technical feasibility. Support is given along the entire value chain of projects: pre-project consultation, project planning, training, sample preparation, microscopy and data analysis.Since start in 2016, NMI has supported more than 1000 researchers in over 1400 research projects. NMI is thus well-established as an infrastructure of relevance for current and future Swedish research.

NMI is a distributed national infrastructure for microscopy in life sciences with nodes located at KTH, and Stockholm, Gothenburg, Umeå and Uppsala Universities. The aim of NMI is to provide access to state-of the-art equipment and expertise in microscopy and image analysis.Technologies available at NMI include: correlative microscopy, multimodal microscopy, intravital microscopy, multiplex high-content microscopy and super-resolution microscopy. The techniques are used for fundamental and translational research to image at molecular to cellular resolutions in single cells to whole organisms. Those advanced imaging techniques require both specialized equipment and expert scientific staff.In this proposal NMI ask to upgrade two super resolution microscopes at KTH and GU to state-of-the-art and to upgrade the intra-vital imaging capacity at SU.The present super-resolution microscopes in NMI are of the first generation, introduced in 2010. They have reached their economical, technical and scientific lifetime. To answer to the requests from users for state-of-the-art equipment and scientific support we need to upgrade those microscopes with the latest developments. This gives new functionality with significantly higher performance in both temporal and spatial resolution and also access to live cell super-resolution imaging, a technique many users have expressed a strong interest for.A small animal fluorescence and luminescence imaging system, IVIS, enhance the capacity for intra-vital imaging. The system allows pre-screening animals prior to preparation with window implementation and high-resolution intra vital imaging. Thereby we can improve the use of each animal specimen according to the 3R (Replace, Reduce, Refine) principle.Since start in 2016, NMI has supported more than 1100 research projects from 760 users with imaging and analysis. NMI is thus established as national infrastructure of importance for current and future Swedish research.