BFC - Flow Cytometry Analysis
Flow Cytometry Analysis involves using a flow cytometer to interrogate cells stained with fluorescent markers and classifying them into groups. Cells pass through the instrument once and go straight to a waste container. There is no cell recovery.
At BFC, flow cytometry analysis can be done either as assisted analysis or as a self-user:
Assisted analysis
Staff members of the facility can acquire samples for non-trained users or for users requiring it.
Please, refer to iLab and create a Work Request under the Request Services tab.
Once accepted, a timeslot can be booked in the instrument麓s calendar.
Self-user analysis
If your project requires the recurrent usage of the analyzers, a training session can be requested. For analysis instruments a driver鈥檚 license can be obtained either by:
- Demonstrating previous experience on a particular instrument
- By getting trained in the facility
Request training
To request training for our analyzers:
- Create a Training request in iLab (Request Services > Introduction and training request: Analyzers).
- The training consists of a hands-on session (1-3,5 h, depending on the instrument), where one of our members will train you on our SOPs for using the instrument. If it is your first time using our facility, this training will include a General introduction to the BFC.
- After the training session, 0-3 assisted analyses must be completed, depending on the user麓s previous experience.
- Once the training session and assisted analysis are completed, you are qualified as a self-user.