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BFC - Flow Cytometry Sorting

Flow Cytometry Sorting involves using a flow cytometer with cell sorting capability to interrogate cells stained with fluorescent markers, classifying them into groups, and then physically separate the groups into test tubes or multi-well plates.

At BFC, the access to our cell sorters can be done either as as a self-user or operated assisted sort:

Workflow sorting for different kinds of users/clients (assisted or self-user sorting).
Biomedicum Flow Cytometry Core Facility, workflow sorting. Photo: BFC

Assisted sort

Staff members of the facility can sort samples for non-trained users or for users requiring it. Please, refer to iLab and under the Request Services tab. Once accepted, we can arrange a pre-sort meeting to discuss matters of the sort. The next step will be for you or our staff to book a timeslot in the instrument麓s calendar at your convenience.

Self-user sort

If your project requires the recurrent usage of the sorters, a training course can be requested. In order to obtain a driver麓s license in our cell sorters, there are two options: 

  • Demonstrating previous experience on a particular Aria sorter. 
  • By getting trained in the facility

Request a spot

In order to request a spot in our Aria Sorting training courses:

  1. (Request Services > Introduction and training request: Sorters). 
  2. The training consists of 5 h theory and 6 h hands-on training, where you are trained on different aspects of using the instrument.. 
  3. After these training sessions, each user will arrange 3 (or more) sessions of individual practice, to gain independence and feel confident to take the Driver麓s License Test, 
  4. The Driver麓s License Test will enable users to have complete flexibility and access to self-operated and out-of-hours sorts.
Content reviewer:
16-07-2024