Rafael Galupa, Life-Science PhD
Degree from KI: MSc Biomedicine
Current post: Post-doc at European Molecular Biology Laboratory (EMBL), at the Heidelberg site in Germany.

What is your background/education experience?
I completed my bachelor in Genetics and Molecular Biology in the University of Lisbon. I wanted to get more hands-on experience in a lab before deciding to follow an academic career, so I applied to a Leonardo Da Vinci fellowship to do an internship abroad, which took me to the Institut Curie, in Paris. There I investigated the role of a specific gene in a subtype of breast cancer, in which cells invert their apicobasal polarity. This left me willing to learn more about the biology of the human body!
How and why did you choose to study at KI?
I wanted to learn more about human biology and I started looking for master's degrees across Europe. I have to say I was a bit late with my application already, because initially I thought I'd stay in Paris for my master's but decided last minute that I should go for an English-speaking experience. When I stumbled across the webpage of KI's MSc in Biomedicine, I immediately fell in love. It combined all the subjects I wanted to learn more about, cancer biology and neurosciences for instance, plus subjects on ethics and science communication, which I felt would be very enriching to 鈥渂uild鈥 myself as a scientist, plus a very international environment! I was very thrilled when I got accepted, I felt it was an unparalleled opportunity.
Describe your experience, including any highlights from your time at KI
My time at KI was a very special one, both in terms of science and life experience. The class was composed of people from all over the world, and getting to know them all was the most fantastic experience I鈥檝e ever had 鈥 I still have very good, close friends from that time. At the master鈥檚, I was impressed by how everything was so organized and how our feedback on how things were run was taken into account almost immediately. I also loved that it was structured in modules and we would do one at a time, mostly 鈥 this was new to me, and I felt it gave us time to fully dive into each topic instead of having to follow several in parallel. I was also lucky to be welcome in Margareta Wilhelm鈥檚 lab, so I gained a lot of lab experience as well while studying!
After you graduated from KI, what was your next step, and how did you find this opportunity?
I chose to go back to Paris for my master鈥檚 thesis, under an Erasmus+ opportunity, to join Edith Heard鈥檚 lab working on X-chromosome inactivation 鈥 back to fundamental research! I knew of this lab from my previous experience in Paris, I heard great things about Edith as a scientist and as a person, and so I decided to apply. It was a fantastic experience, I was in one of the best labs in the world in the topic, surrounded by very inspiring people. Plus, new techniques were rapidly being implemented 鈥 this was early 2013 and I still remember preparing CRISPR plasmids that had just arrived in the lab! I had the opportunity to set up this system in the lab and try it out myself in mouse embryonic stem cells. When I was invited to stay for a PhD, I didn鈥檛 think twice!
What is your current role?
Currently I鈥檓 finishing my postdoc at the European Molecular Biology Laboratory (EMBL), at the Heidelberg site in Germany, in Justin Crocker鈥檚 lab. Here I鈥檓 still working with developmental processes and gene regulation, but in the fly embryo. I knew Justin鈥檚 work from papers, which I really liked because of the combination between experiments and theoretical models. When I heard he was starting his lab at EMBL, I thought this was a great opportunity, also to have the experience of being in a starting lab, so I tried my luck with the EMBL鈥檚 postdoctoral programme, which awards fellowships for three years, and got in.
Describe a day on the job.
At the moment I am supervising an undergraduate student, so often the day starts with catching up with her on how things went the previous day and set a plan for the new day. I also try to briefly catch up with emails in the morning (I almost never manage) and then I have flies and experiments to attend to 鈥 setting up new crosses, starting new stainings of fly embryos, maybe going to the microscope to look at those stainings. Fly embryos are always gorgeous to look at! Often I have one or two meetings per day as well 鈥 either with a collaborator, or lab-related, or department-related. I鈥檓 also involved in some institutional initiatives, related to mental health and to equality, diversity and inclusion. Never one day is alike another, and that鈥檚 what I really enjoy as well!
How did your studies at KI help your future career?
My studies at KI gave me a breadth of knowledge that I am very thankful for 鈥 on the one hand in terms of scientific topics, on the other hand in terms of skills, either laboratory, communication, critical thinking or so-called soft skills. Even if I鈥檓 not directly working with 鈥渉uman biology鈥 anymore, I feel advantaged for having studied Biomedicine, which allows me to better follow certain seminars or sometimes make connections in my work with things that I learnt previously. The internships in Maggan鈥檚 lab (at MTC) were also critical, because they let me develop independence and maturity in the lab 鈥 when I moved on to my PhD, I felt I was already very at ease being in a lab and moving on with my work.
What are your future aspirations?
I鈥檓 in the final stages of my postdoc, and I鈥檓 looking for opportunities to set up my own line of research and have my own lab. I would like to go back to research on the X chromosome, as I felt so many unanswered questions stayed with me. So I would like to set up a lab known for its rigorous and solid research, significantly contributing to the field and where people are happy to work and feel that they are developing as scientists and as human beings. I would also like to combine research with teaching 鈥 I really enjoy the interaction with students, so I would like to be a professor one day!
What is your advice to current students?
What worked for me at each step was to go after what I felt like I wanted to learn, what I felt like would be a significant experience for my development as a scientist and as a person. So my advice would be for each of us, every now and then, to take time for ourselves to figure out what it is that we want to be doing 鈥 and question this from time to time too. Am I still enjoying myself? Would I rather be doing something else? I鈥檓 always questioning myself, and I think that鈥檚 a good thing, because it allows me to stay in tune with what I want and because it either reinforces my previous decisions or it shows me that I need to think about them differently.